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SKU(재고 관리 코드):EH10975

Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit

Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit

Size
Sensitivity
Range

Product Name

Human PDE4A(cAMP-specific 3′,5′-cyclic phosphodiesterase 4A)ELISA Kit

Alias

cAMP-specific 3',5'-cyclic phosphodiesterase 4A ELISA KitDPDE2 ELISA KitPDE46 ELISA KitPDE4A ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PDE4AELISA Kit allows for the in vitro quantitative determination of PDE4A concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Human

UniProt ID

P27815

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDE4A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4A in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-104

94

EDTA plasma(n=5)

88-101

93

heparin plasma(n=5)

88-94

91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-104%

89-102%

87-104%

EDTA plasma(n=5)

85-100%

84-96%

84-101%

heparin plasma(n=5)

83-92%

86-100%

85-92%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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