FineTest
SKU(재고 관리 코드):EH10975
Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit
Human PDE4A(cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit
Product Name
Human PDE4A(cAMP-specific 3′,5′-cyclic phosphodiesterase 4A)ELISA Kit
Alias
cAMP-specific 3',5'-cyclic phosphodiesterase 4A ELISA Kit, DPDE2 ELISA Kit, PDE46 ELISA Kit, PDE4A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDE4AELISA Kit allows for the in vitro quantitative determination of PDE4A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDE4A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
94 |
|
EDTA plasma(n=5) |
88-101 |
93 |
|
heparin plasma(n=5) |
88-94 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-104% |
89-102% |
87-104% |
|
EDTA plasma(n=5) |
85-100% |
84-96% |
84-101% |
|
heparin plasma(n=5) |
83-92% |
86-100% |
85-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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