FineTest
SKU(재고 관리 코드):EH11652
Human RAF1 ELISA Kit
Human RAF1 ELISA Kit
Product Name
Human RAF1 ELISA Kit
Alias
RAF proto-oncogene serine/threonine-protein kinase ELISA Kit, Proto-oncogene c-RAF (cRaf) ELISA Kit, Raf-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RAF1 ELISA Kit allows for the in vitro quantitative determination of RAF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RAF1 and the recovery rates were calculated by comparing the measured value to the expected amount of RAF1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
96 |
|
EDTA plasma(n=5) |
91-101 |
97 |
|
heparin plasma(n=5) |
91-99 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-102% |
86-103% |
85-100% |
|
EDTA plasma(n=5) |
83-95% |
84-98% |
89-101% |
|
heparin plasma(n=5) |
86-96% |
80-95% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
