FineTest
SKU(재고 관리 코드):EU2620
VB12(Vitamin B12)ELISA Kit
VB12(Vitamin B12)ELISA Kit
Product Name
VB12 (Vitamin B12) ELISA Kit
Alias
Vitamin B12 ELISA Kit, VB12 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
VB12 ELISA Kit allows for the in vitro quantitative determination of VB12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
50-500pg/ml
Sensitivity
< 25pg/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VB12 and the recovery rates were calculated by comparing the measured value to the expected amount of VB12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
95 |
|
EDTA plasma(n=5) |
81-102 |
94 |
|
heparin plasma(n=5) |
82-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VB12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
80-96% |
87-99% |
94-103% |
|
EDTA plasma(n=5) |
92-101% |
90-96% |
92-100% |
|
heparin plasma(n=5) |
93-99% |
91-99% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
