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FineTest

EM2085

Mouse GCLC(Glutamate-cysteine ligase catalytic subunit)ELISA Kit

Mouse GCLC(Glutamate-cysteine ligase catalytic subunit)ELISA Kit

Size
Sensitivity
Range

Product Name

Mouse GCLC(Glutamate-cysteine ligase catalytic subunit) ELISA Kit 

Alias

GCLC ELISA Kit, Glutamate-cysteine ligase catalytic subunit ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

GCLC ELISA Kit  allows for the in vitro quantitative determination of GCLC concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Mouse

UniProt ID

P97494

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GCLC and the recovery rates were calculated by comparing the measured value to the expected amount of GCLC in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-102

94

EDTA plasma(n=5)

91-97

95

heparin plasma(n=5)

85-101

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCLC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

89-100%

87-102%

91-103%

EDTA plasma(n=5)

83-99%

82-100%

87-101%

heparin plasma(n=5)

85-98%

82-96%

81-98%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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