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Mouse ISG15(Ubiquitin-like protein ISG15) ELISA Kit

Mouse ISG15(Ubiquitin-like protein ISG15) ELISA Kit

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Size
Sensitivity
Range

Product Name

Mouse ISG15(Ubiquitin-like protein ISG15) ELISA Kit 

Alias

ISG15 ELISA Kit, Ubiquitin-like protein ISG15 ELISA Kit, Interferon-induced 15 kDa protein ELISA Kit, Ubiquitin cross-reactive protein ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

ISG15 ELISA Kit  allows for the in vitro quantitative determination of ISG15 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Mouse

UniProt ID

Q64339

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ISG15 and the recovery rates were calculated by comparing the measured value to the expected amount of ISG15 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-105

94

EDTA plasma(n=5)

87-104

93

heparin plasma(n=5)

88-105

96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ISG15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

94-105%

93-104%

93-102%

EDTA plasma(n=5)

87-95%

85-101%

82-101%

heparin plasma(n=5)

91-100%

86-92%

80-98%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only


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