Uncoated Human HSP-70/HSPA9(Heat Shock 70 kDa Protein 9) ELISA Kit

The Uncoated Human HSP-70/HSPA9 ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of HSP-70/HSPA9 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of HSP-70/HSPA9. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample to the microplate wells, followed by the addition of the detection antibody-HRP conjugate. After incubation and washing, a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of HSP-70/HSPA9 present in the sample.

This kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. It has a high degree of accuracy and reproducibility, with intra-assay and inter-assay coefficients of variation (CVs) of less than 10%. The kit is easy to use, with a simple protocol that can be completed in less than 3 hours. It is suitable for use in research and clinical applications, including the study of HSP-70/HSPA9 expression in various diseases and conditions, such as cancer, neurodegenerative disorders, and cardiovascular disease. Overall, the Uncoated Human HSP-70/HSPA9 ELISA Kit is a reliable and effective tool for the quantitative measurement of HSP-70/HSPA9 in human samples.