Uncoated Human ANXA1(Annexin A1) ELISA Kit

The Uncoated Human ANXA1(Annexin A1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of human ANXA1 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of ANXA1. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP) and used for detection. The uncoated format of this kit allows for greater flexibility in assay design and optimization, making it ideal for researchers who require a customizable assay for their specific needs.

The Uncoated Human ANXA1 ELISA Kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. The assay has a high degree of accuracy and reproducibility, with intra-assay and inter-assay coefficients of variation (CVs) of less than 10%. The kit includes all necessary reagents and components for the assay, including microplate wells, standards, detection antibody, HRP-conjugated secondary antibody, and substrate solution. The assay protocol is simple and straightforward, with a total assay time of approximately 3 hours. This kit is an essential tool for researchers studying the role of ANXA1 in various biological processes, including inflammation, apoptosis, and cancer.