Uncoated Human VWF(Von Willebrand Factor) ELISA Kit

The Uncoated Human VWF (Von Willebrand Factor) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of human VWF in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of the VWF molecule. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP) and added to the sample. The VWF in the sample binds to the capture antibody, and the detection antibody-HRP conjugate binds to the VWF, forming a sandwich complex. After washing away any unbound materials, a substrate solution is added, which reacts with the HRP to produce a colorimetric signal that is proportional to the amount of VWF in the sample.

This kit has a wide dynamic range of detection, from 0.156 ng/mL to 10 ng/mL, and a low limit of detection (LOD) of 0.078 ng/mL. It has excellent precision and accuracy, with intra-assay and inter-assay coefficients of variation (CVs) less than 10%. The kit is easy to use, with a simple protocol that can be completed in less than 3 hours. It is suitable for research and clinical applications, including the diagnosis and monitoring of VWF-related disorders such as von Willebrand disease, thrombotic thrombocytopenic purpura, and hemolytic uremic syndrome. The Uncoated Human VWF ELISA Kit is a reliable and cost-effective tool for measuring VWF levels in human samples.