Uncoated Human FG(Fibrinogen) ELISA Kit

The Uncoated Human FG (Fibrinogen) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of human fibrinogen in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of the target protein. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample to the microplate wells, followed by the addition of the detection antibody-HRP conjugate. After incubation and washing steps, a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of fibrinogen present in the sample.

This kit is uncoated, which means that it does not require any pre-coating of the microplate wells with the capture antibody. This simplifies the assay procedure and reduces the assay time, while maintaining high sensitivity and specificity. The kit has a wide dynamic range of detection, from 0.156 to 10 ng/mL, and a low limit of detection of 0.078 ng/mL. It is suitable for use in research and clinical laboratories for the diagnosis and monitoring of various diseases and conditions, such as cardiovascular disease, liver disease, and cancer. The kit is supplied with all the necessary reagents and components, including the microplate, standards, detection antibody-HRP conjugate, substrate solution, and stop solution, as well as a detailed protocol for performing the assay.