Uncoated Human tPA(Tissue-type Plasminogen Activator) ELISA Kit

The Uncoated Human tPA ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Tissue-type Plasminogen Activator (tPA) in human serum, plasma, and cell culture supernatants. The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two highly specific antibodies that recognize different epitopes of tPA. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample to the microplate wells, followed by the addition of the detection antibody-HRP conjugate. After incubation and washing steps, a substrate solution is added, which reacts with the HRP to produce a colorimetric signal that is proportional to the amount of tPA present in the sample.

The Uncoated Human tPA ELISA Kit is a reliable and accurate tool for the detection of tPA in various biological samples. The kit has a wide dynamic range of detection, with a lower limit of detection of 0.156 ng/mL and an upper limit of 10 ng/mL. The assay is highly reproducible, with intra-assay and inter-assay coefficients of variation of less than 10%. The kit is easy to use, with a simple protocol that can be completed in less than 3 hours. The Uncoated Human tPA ELISA Kit is an essential tool for researchers studying the role of tPA in various physiological and pathological processes, including thrombosis, stroke, and cancer.