Uncoated Mouse RANTES(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

The Uncoated Mouse RANTES ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of RANTES in mouse serum, plasma, and cell culture supernatants. RANTES, also known as CCL5, is a chemokine that plays a crucial role in the recruitment and activation of leukocytes during inflammation and immune responses. The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, in which RANTES is captured by a monoclonal antibody coated on the microplate wells and detected by a biotinylated polyclonal antibody followed by streptavidin-HRP conjugate. The assay has a detection range of 15.6-1000 pg/mL and a sensitivity of 7.8 pg/mL.

The Uncoated Mouse RANTES ELISA Kit is uncoated, meaning that the microplate wells are not pre-coated with any blocking agents or capture antibodies. This allows for greater flexibility in assay design and optimization, as well as reduced background noise and interference. The kit includes all necessary reagents and components, including standards, controls, and buffers, for the accurate and reproducible quantification of RANTES in mouse samples. The assay is easy to perform, with a total assay time of approximately 3 hours, and requires only basic laboratory equipment. The Uncoated Mouse RANTES ELISA Kit is a valuable tool for researchers studying the role of RANTES in inflammation, immune responses, and disease pathogenesis in mouse models.