Uncoated Mouse CFH(Complement Factor H) ELISA Kit

The Uncoated Mouse CFH ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of complement factor H (CFH) in mouse serum, plasma, and other biological fluids. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and components for the assay, including a pre-coated standard curve, detection antibody, streptavidin-HRP conjugate, and TMB substrate solution.

The assay is based on a sandwich ELISA format, in which CFH in the sample is captured by a specific antibody coated onto the microplate, and then detected using a biotinylated detection antibody and streptavidin-HRP conjugate. The TMB substrate solution is added to the wells, resulting in a colorimetric reaction that is proportional to the amount of CFH present in the sample. The assay has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. The kit is easy to use and provides accurate and reproducible results, making it an ideal tool for researchers studying the role of CFH in various biological processes and disease states.