Uncoated Mouse PDCD1(Programmed Cell Death Protein 1) ELISA Kit

The Uncoated Mouse PDCD1 ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of programmed cell death protein 1 (PDCD1) in mouse serum, plasma, and cell culture supernatants. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and components for the assay, including a pre-coated detection antibody, standards, and controls.

The assay is based on a sandwich ELISA format, in which PDCD1 is captured by the immobilized detection antibody and detected by a biotinylated detection antibody and streptavidin-HRP conjugate. The kit has a detection range of 31.25-2000 pg/mL, with a sensitivity of 18.75 pg/mL. The assay has a high degree of accuracy and reproducibility, with intra-assay and inter-assay coefficients of variation of less than 10% and 15%, respectively. The kit is easy to use and can be completed in less than 4 hours, making it an ideal tool for high-throughput screening and research applications.