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Uncoated Mouse CCL12/MCP-5(Monocyte Chemotactic Protein 5) ELISA Kit
Uncoated Mouse CCL12/MCP-5(Monocyte Chemotactic Protein 5) ELISA Kit
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The Uncoated Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of CCL12/MCP-5 in mouse serum, plasma, and other biological samples. This ELISA kit is specifically developed to provide accurate and sensitive results, enabling researchers to study the role of CCL12/MCP-5 in various biological processes and diseases.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high specificity and sensitivity. The assay is based on the principle of the solid-phase enzyme immunoassay, where CCL12/MCP-5 present in the sample binds to the pre-coated monoclonal antibody. After a series of washing steps to remove any unbound substances, a biotin-conjugated polyclonal antibody specific to CCL12/MCP-5 is added, forming a sandwich complex. This complex is then incubated with streptavidin-conjugated horseradish peroxidase (HRP), which catalyzes a colorimetric reaction upon the addition of a substrate solution. The intensity of the color developed is directly proportional to the concentration of CCL12/MCP-5 in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
The Uncoated Mouse CCL12/MCP-5 ELISA Kit offers several advantages, including its wide dynamic range, high sensitivity (with a lower limit of detection of 0.1 ng/mL), and excellent reproducibility. It provides a user-friendly protocol, with all necessary reagents and materials included in the kit. The kit is suitable for both experienced researchers and beginners in the field, allowing for accurate and precise quantification of CCL12/MCP-5 levels in various biological samples. With its exceptional performance, this ELISA kit is an indispensable tool for investigating the role of CCL12/MCP-5 in inflammation, immune response, and other related research areas.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high specificity and sensitivity. The assay is based on the principle of the solid-phase enzyme immunoassay, where CCL12/MCP-5 present in the sample binds to the pre-coated monoclonal antibody. After a series of washing steps to remove any unbound substances, a biotin-conjugated polyclonal antibody specific to CCL12/MCP-5 is added, forming a sandwich complex. This complex is then incubated with streptavidin-conjugated horseradish peroxidase (HRP), which catalyzes a colorimetric reaction upon the addition of a substrate solution. The intensity of the color developed is directly proportional to the concentration of CCL12/MCP-5 in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
The Uncoated Mouse CCL12/MCP-5 ELISA Kit offers several advantages, including its wide dynamic range, high sensitivity (with a lower limit of detection of 0.1 ng/mL), and excellent reproducibility. It provides a user-friendly protocol, with all necessary reagents and materials included in the kit. The kit is suitable for both experienced researchers and beginners in the field, allowing for accurate and precise quantification of CCL12/MCP-5 levels in various biological samples. With its exceptional performance, this ELISA kit is an indispensable tool for investigating the role of CCL12/MCP-5 in inflammation, immune response, and other related research areas.
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