1
/
의
1
Elabscience
SKU(재고 관리 코드):E-UNEL-M0048
Uncoated Mouse ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
Uncoated Mouse ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
The Uncoated Mouse ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of ESM1 in mouse serum, plasma, and other biological samples. This ELISA kit is specifically developed to detect and quantify ESM1, a protein that plays a crucial role in endothelial cell function and angiogenesis.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high sensitivity and specificity. The assay is performed in a 96-well plate coated with a monoclonal antibody specific to ESM1. The uncoated format of this kit allows for flexibility in sample types, making it suitable for a wide range of applications. The assay procedure involves the addition of samples, standards, and controls to the wells, followed by the addition of a biotin-conjugated antibody specific to ESM1. After a thorough washing step, an enzyme-conjugated streptavidin is added, which binds to the biotinylated antibody. Finally, a substrate solution is added, resulting in a color change that is proportional to the amount of ESM1 present in the sample. The absorbance is then measured using a microplate reader, and the concentration of ESM1 in the sample is determined by comparing it to a standard curve.
This Uncoated Mouse ESM1 ELISA Kit offers several advantages, including its high sensitivity, wide dynamic range, and ease of use. It provides accurate and reproducible results, making it an essential tool for researchers studying endothelial cell biology, angiogenesis, and related fields. With its comprehensive instructions and all necessary components included, this kit offers a convenient and reliable solution for the quantitative measurement of ESM1 in mouse samples.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high sensitivity and specificity. The assay is performed in a 96-well plate coated with a monoclonal antibody specific to ESM1. The uncoated format of this kit allows for flexibility in sample types, making it suitable for a wide range of applications. The assay procedure involves the addition of samples, standards, and controls to the wells, followed by the addition of a biotin-conjugated antibody specific to ESM1. After a thorough washing step, an enzyme-conjugated streptavidin is added, which binds to the biotinylated antibody. Finally, a substrate solution is added, resulting in a color change that is proportional to the amount of ESM1 present in the sample. The absorbance is then measured using a microplate reader, and the concentration of ESM1 in the sample is determined by comparing it to a standard curve.
This Uncoated Mouse ESM1 ELISA Kit offers several advantages, including its high sensitivity, wide dynamic range, and ease of use. It provides accurate and reproducible results, making it an essential tool for researchers studying endothelial cell biology, angiogenesis, and related fields. With its comprehensive instructions and all necessary components included, this kit offers a convenient and reliable solution for the quantitative measurement of ESM1 in mouse samples.
Share
