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Elabscience
SKU(재고 관리 코드):E-UNEL-M0067
Uncoated Mouse OPN(Osteopontin) ELISA Kit
Uncoated Mouse OPN(Osteopontin) ELISA Kit
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The Uncoated Mouse OPN (Osteopontin) ELISA Kit is a cutting-edge and reliable tool designed for the accurate measurement of Osteopontin levels in mouse samples. This ELISA kit is specifically developed to provide researchers with a comprehensive and efficient solution for studying the role of Osteopontin in various biological processes and disease conditions.
This kit utilizes a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of Osteopontin in a wide range of sample types, including serum, plasma, and cell culture supernatants. The assay is based on the principle of a solid-phase sandwich ELISA, where a monoclonal antibody specific to Osteopontin is pre-coated onto the microplate wells. Upon sample addition, Osteopontin present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody specific to Osteopontin is then added, forming a sandwich complex. After another washing step, an enzyme-conjugated streptavidin is added, which binds to the biotinylated antibody. Finally, a substrate solution is added, resulting in the development of a blue color. The intensity of this color is directly proportional to the amount of Osteopontin present in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
With its high sensitivity, wide dynamic range, and excellent reproducibility, the Uncoated Mouse OPN ELISA Kit offers researchers a powerful tool for investigating the role of Osteopontin in various physiological and pathological processes. This kit provides accurate and precise results, enabling researchers to obtain valuable insights into the molecular mechanisms underlying Osteopontin-related diseases, such as cancer, inflammation, and cardiovascular disorders. Furthermore, the kit is user-friendly, with a simple and straightforward protocol that can be completed within a few hours. Its robust performance and ease of use make it an indispensable tool for any laboratory conducting research in the field of Osteopontin biology.
This kit utilizes a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of Osteopontin in a wide range of sample types, including serum, plasma, and cell culture supernatants. The assay is based on the principle of a solid-phase sandwich ELISA, where a monoclonal antibody specific to Osteopontin is pre-coated onto the microplate wells. Upon sample addition, Osteopontin present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody specific to Osteopontin is then added, forming a sandwich complex. After another washing step, an enzyme-conjugated streptavidin is added, which binds to the biotinylated antibody. Finally, a substrate solution is added, resulting in the development of a blue color. The intensity of this color is directly proportional to the amount of Osteopontin present in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
With its high sensitivity, wide dynamic range, and excellent reproducibility, the Uncoated Mouse OPN ELISA Kit offers researchers a powerful tool for investigating the role of Osteopontin in various physiological and pathological processes. This kit provides accurate and precise results, enabling researchers to obtain valuable insights into the molecular mechanisms underlying Osteopontin-related diseases, such as cancer, inflammation, and cardiovascular disorders. Furthermore, the kit is user-friendly, with a simple and straightforward protocol that can be completed within a few hours. Its robust performance and ease of use make it an indispensable tool for any laboratory conducting research in the field of Osteopontin biology.
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