Uncoated Mouse HGF(Hepatocyte Growth Factor) ELISA Kit

The Uncoated Mouse HGF (Hepatocyte Growth Factor) ELISA Kit is a highly sensitive and specific assay designed for the quantitative determination of HGF in mouse serum, plasma, and cell culture supernatants. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and materials for the assay, including a pre-coated standard curve, detection antibody, streptavidin-HRP conjugate, and TMB substrate solution.

The assay is based on a sandwich ELISA principle, in which HGF in the sample is captured by a specific antibody immobilized on the microplate, and then detected by a second antibody conjugated to horseradish peroxidase (HRP). The HRP catalyzes the conversion of the TMB substrate to a blue color, which turns yellow upon addition of the stop solution. The intensity of the yellow color is proportional to the amount of HGF in the sample, and can be measured using a microplate reader at 450 nm. The assay has a detection range of 31.25-2000 pg/mL, with a sensitivity of 18.75 pg/mL. This kit is ideal for researchers studying the role of HGF in various biological processes, including cell proliferation, differentiation, and migration, as well as in disease states such as cancer and liver regeneration.