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Elabscience
SKU(재고 관리 코드):E-UNEL-M0107
Uncoated Mouse DPP4(Dipeptidyl Peptidase Ⅳ) ELISA Kit
Uncoated Mouse DPP4(Dipeptidyl Peptidase Ⅳ) ELISA Kit
The Uncoated Mouse DPP4 (Dipeptidyl Peptidase IV) ELISA Kit is a cutting-edge and reliable tool designed for the accurate and sensitive measurement of DPP4 levels in mouse samples. This ELISA kit is specifically developed to cater to the needs of researchers and scientists working in the field of immunology, diabetes, and metabolic disorders.
This kit utilizes a solid-phase enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of DPP4 in a variety of biological samples, including serum, plasma, and tissue homogenates. The assay is based on the principle of a sandwich immunoassay, where a monoclonal antibody specific to mouse DPP4 is pre-coated onto the microplate wells. Upon sample addition, DPP4 present in the sample binds to the immobilized antibody, forming an antigen-antibody complex. This complex is then detected using a biotinylated detection antibody and streptavidin-conjugated horseradish peroxidase (HRP), followed by the addition of a chromogenic substrate. The intensity of the color developed is directly proportional to the concentration of DPP4 in the sample, which can be measured spectrophotometrically at 450 nm.
The Uncoated Mouse DPP4 ELISA Kit offers several advantages, including its high sensitivity, specificity, and reproducibility. With a detection range of 0.156-10 ng/mL, this kit allows for the precise quantification of DPP4 levels in a wide range of concentrations. Furthermore, the kit provides all the necessary components, including pre-coated microplate wells, standards, detection antibodies, streptavidin-HRP, and chromogenic substrate, ensuring convenience and ease of use. The detailed protocol provided with the kit offers step-by-step instructions, enabling researchers to perform the assay with minimal effort and obtain accurate and reliable results. Overall, the Uncoated Mouse DPP4 ELISA Kit is an indispensable tool for studying the role of DPP4 in various physiological and pathological processes, contributing to advancements in the field of biomedical research.
This kit utilizes a solid-phase enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of DPP4 in a variety of biological samples, including serum, plasma, and tissue homogenates. The assay is based on the principle of a sandwich immunoassay, where a monoclonal antibody specific to mouse DPP4 is pre-coated onto the microplate wells. Upon sample addition, DPP4 present in the sample binds to the immobilized antibody, forming an antigen-antibody complex. This complex is then detected using a biotinylated detection antibody and streptavidin-conjugated horseradish peroxidase (HRP), followed by the addition of a chromogenic substrate. The intensity of the color developed is directly proportional to the concentration of DPP4 in the sample, which can be measured spectrophotometrically at 450 nm.
The Uncoated Mouse DPP4 ELISA Kit offers several advantages, including its high sensitivity, specificity, and reproducibility. With a detection range of 0.156-10 ng/mL, this kit allows for the precise quantification of DPP4 levels in a wide range of concentrations. Furthermore, the kit provides all the necessary components, including pre-coated microplate wells, standards, detection antibodies, streptavidin-HRP, and chromogenic substrate, ensuring convenience and ease of use. The detailed protocol provided with the kit offers step-by-step instructions, enabling researchers to perform the assay with minimal effort and obtain accurate and reliable results. Overall, the Uncoated Mouse DPP4 ELISA Kit is an indispensable tool for studying the role of DPP4 in various physiological and pathological processes, contributing to advancements in the field of biomedical research.
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