Uncoated Mouse GRN(Granulin) ELISA Kit

The Uncoated Mouse GRN (Granulin) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of mouse GRN in serum, plasma, and cell culture supernatants. The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of the target protein. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample, standards, and detection reagent to the microplate wells, followed by incubation and washing steps. The HRP substrate is then added, and the color development is measured using a microplate reader.

The Uncoated Mouse GRN ELISA Kit has a wide dynamic range of detection (0.156-10 ng/mL) and a low limit of detection (0.078 ng/mL). The kit has been validated for use with mouse serum, plasma, and cell culture supernatants, and has been shown to have high accuracy and precision. The kit is easy to use and requires only a small sample volume (50 µL). The kit includes all necessary reagents and a detailed protocol, making it a convenient and reliable tool for researchers studying the role of GRN in various biological processes.