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Elabscience
SKU(재고 관리 코드):E-UNEL-M0102
Uncoated Mouse RBP4(Retinol Binding Protein 4) ELISA Kit
Uncoated Mouse RBP4(Retinol Binding Protein 4) ELISA Kit
The Uncoated Mouse RBP4 (Retinol Binding Protein 4) ELISA Kit is a cutting-edge and reliable tool designed for the accurate measurement of RBP4 levels in mouse samples. This ELISA kit is specifically developed to provide researchers with a comprehensive and efficient solution for studying retinol metabolism and its associated disorders.
This kit utilizes a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of RBP4 in a wide range of sample types, including serum, plasma, and tissue homogenates. The assay is based on the principle of a solid-phase sandwich ELISA, where a monoclonal antibody specific to mouse RBP4 is pre-coated onto the microplate wells. Upon sample addition, RBP4 present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody is then added, forming a sandwich complex with the captured RBP4. This complex is further detected by the addition of streptavidin-conjugated horseradish peroxidase (HRP), followed by the substrate solution. The enzymatic reaction catalyzed by HRP generates a blue color, which turns yellow upon the addition of the stop solution. The intensity of the color is directly proportional to the concentration of RBP4 in the sample, which can be measured spectrophotometrically at 450 nm.
With its high sensitivity and specificity, this Uncoated Mouse RBP4 ELISA Kit offers researchers a reliable and accurate tool for quantifying RBP4 levels in mouse samples. It provides a wide dynamic range, allowing for the detection of RBP4 concentrations as low as 0.156 ng/mL and as high as 10 ng/mL. The kit includes all the necessary components, including pre-coated microplate wells, standards, controls, detection antibodies, streptavidin-HRP, substrate solution, and stop solution, ensuring convenience and ease of use. Additionally, the kit comes with a detailed protocol, guiding researchers through the entire assay procedure. Whether studying retinol metabolism, investigating the role of RBP4 in disease pathogenesis, or exploring potential therapeutic interventions, the Uncoated Mouse RBP4 ELISA Kit is an indispensable tool for any researcher in the field.
This kit utilizes a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) technique, which allows for the quantitative detection of RBP4 in a wide range of sample types, including serum, plasma, and tissue homogenates. The assay is based on the principle of a solid-phase sandwich ELISA, where a monoclonal antibody specific to mouse RBP4 is pre-coated onto the microplate wells. Upon sample addition, RBP4 present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody is then added, forming a sandwich complex with the captured RBP4. This complex is further detected by the addition of streptavidin-conjugated horseradish peroxidase (HRP), followed by the substrate solution. The enzymatic reaction catalyzed by HRP generates a blue color, which turns yellow upon the addition of the stop solution. The intensity of the color is directly proportional to the concentration of RBP4 in the sample, which can be measured spectrophotometrically at 450 nm.
With its high sensitivity and specificity, this Uncoated Mouse RBP4 ELISA Kit offers researchers a reliable and accurate tool for quantifying RBP4 levels in mouse samples. It provides a wide dynamic range, allowing for the detection of RBP4 concentrations as low as 0.156 ng/mL and as high as 10 ng/mL. The kit includes all the necessary components, including pre-coated microplate wells, standards, controls, detection antibodies, streptavidin-HRP, substrate solution, and stop solution, ensuring convenience and ease of use. Additionally, the kit comes with a detailed protocol, guiding researchers through the entire assay procedure. Whether studying retinol metabolism, investigating the role of RBP4 in disease pathogenesis, or exploring potential therapeutic interventions, the Uncoated Mouse RBP4 ELISA Kit is an indispensable tool for any researcher in the field.
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