Uncoated Rat SELL(L-Selectin) ELISA Kit

The Uncoated Rat SELL(L-Selectin) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of rat SELL (L-Selectin) in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific for rat SELL (L-Selectin) coated onto a 96-well plate. The sample is added to the wells, and any SELL (L-Selectin) present is bound by the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific for rat SELL (L-Selectin) is added to the wells. Following a wash to remove any unbound biotinylated antibody, horseradish peroxidase (HRP)-conjugated streptavidin is added to the wells. The wells are then washed to remove any unbound streptavidin-HRP, and a substrate solution is added to the wells. A color develops in proportion to the amount of rat SELL (L-Selectin) bound in the initial step. The color development is stopped, and the intensity of the color is measured.

This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL. It has a high degree of accuracy and reproducibility, with intra-assay and inter-assay coefficients of variation of less than 10%. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours. It is suitable for use in research applications, including the study of inflammation, immune response, and cell adhesion. Overall, the Uncoated Rat SELL(L-Selectin) ELISA Kit is a reliable and efficient tool for the quantitative measurement of rat SELL (L-Selectin) in biological samples.