Uncoated Rat IL-1R1(Interleukin 1 Receptor Type I) ELISA Kit

The Uncoated Rat IL-1R1 ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Interleukin 1 Receptor Type I (IL-1R1) in rat serum, plasma, and cell culture supernatants. The kit is uncoated, which means that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and components for the assay, including a pre-coated standard curve, detection antibody, streptavidin-HRP conjugate, and TMB substrate solution.

The assay is based on a sandwich ELISA format, in which the capture antibody binds to IL-1R1 in the sample, and the detection antibody binds to a different epitope on the same molecule. The streptavidin-HRP conjugate then binds to the detection antibody, and the resulting complex is detected by the addition of TMB substrate solution, which produces a blue color that can be quantified using a microplate reader. The kit has a detection range of 31.25-2000 pg/mL, with a sensitivity of 18.75 pg/mL. The assay is highly reproducible, with intra-assay and inter-assay coefficients of variation of less than 10% and 15%, respectively.

Overall, the Uncoated Rat IL-1R1 ELISA Kit is a reliable and accurate tool for the measurement of IL-1R1 in rat samples, and is suitable for a wide range of applications, including basic research, drug discovery, and clinical diagnostics. The uncoated format allows for greater flexibility in assay design and optimization, and the kit includes all necessary reagents and components for the assay. With its high sensitivity, specificity, and reproducibility, the kit is an essential tool for researchers and clinicians studying the role of IL-1R1 in health and disease.