1
/
의
1
Elabscience
SKU(재고 관리 코드):E-UNEL-R0009
Uncoated Rat EGF(Epidermal Growth Factor) ELISA Kit
Uncoated Rat EGF(Epidermal Growth Factor) ELISA Kit
The Uncoated Rat EGF (Epidermal Growth Factor) ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of Rat EGF in various biological samples. This ELISA kit is specifically developed for research purposes and provides accurate results with high sensitivity. The kit includes all the necessary components, such as pre-coated microplates, standards, controls, and reagents, ensuring a hassle-free and convenient experimental setup.
The Uncoated Rat EGF ELISA Kit utilizes a sandwich enzyme immunoassay technique, which allows for the precise detection and quantification of Rat EGF in a wide range of sample types, including serum, plasma, tissue homogenates, and cell culture supernatants. The assay is based on the principle of antigen-antibody interaction, where the Rat EGF present in the sample binds to the immobilized capture antibody on the microplate wells. After a series of washing steps to remove any unbound substances, a detection antibody conjugated with horseradish peroxidase (HRP) is added, forming a sandwich complex. The addition of a substrate solution triggers a color change, and the intensity of the color is directly proportional to the concentration of Rat EGF in the sample. The absorbance is then measured using a microplate reader, providing precise and quantitative results.
With its high sensitivity and specificity, the Uncoated Rat EGF ELISA Kit is an essential tool for researchers studying the role of Rat EGF in various biological processes, including cell proliferation, differentiation, and tissue regeneration. Its user-friendly design and comprehensive instructions ensure accurate and reproducible results, making it suitable for both experienced researchers and beginners. This ELISA kit offers a cost-effective and time-saving solution for quantitative analysis of Rat EGF, enabling researchers to advance their understanding of the molecular mechanisms underlying various physiological and pathological conditions.
The Uncoated Rat EGF ELISA Kit utilizes a sandwich enzyme immunoassay technique, which allows for the precise detection and quantification of Rat EGF in a wide range of sample types, including serum, plasma, tissue homogenates, and cell culture supernatants. The assay is based on the principle of antigen-antibody interaction, where the Rat EGF present in the sample binds to the immobilized capture antibody on the microplate wells. After a series of washing steps to remove any unbound substances, a detection antibody conjugated with horseradish peroxidase (HRP) is added, forming a sandwich complex. The addition of a substrate solution triggers a color change, and the intensity of the color is directly proportional to the concentration of Rat EGF in the sample. The absorbance is then measured using a microplate reader, providing precise and quantitative results.
With its high sensitivity and specificity, the Uncoated Rat EGF ELISA Kit is an essential tool for researchers studying the role of Rat EGF in various biological processes, including cell proliferation, differentiation, and tissue regeneration. Its user-friendly design and comprehensive instructions ensure accurate and reproducible results, making it suitable for both experienced researchers and beginners. This ELISA kit offers a cost-effective and time-saving solution for quantitative analysis of Rat EGF, enabling researchers to advance their understanding of the molecular mechanisms underlying various physiological and pathological conditions.
Share
