Uncoated Rat IL-1α(Interleukin 1 Alpha) ELISA Kit

The Uncoated Rat IL-1α (Interleukin 1 Alpha) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of rat IL-1α in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of the target protein. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample and the detection antibody-HRP conjugate to the wells, followed by incubation and washing steps. The amount of IL-1α present in the sample is then quantified by measuring the absorbance of the HRP substrate using a microplate reader.

This ELISA kit has a detection range of 15.6-1000 pg/mL and a sensitivity of 7.8 pg/mL. It has been validated for use with rat samples and has a high degree of accuracy and reproducibility. The kit includes all necessary reagents and a detailed protocol for easy and efficient use. It is ideal for researchers studying the role of IL-1α in inflammation, immune response, and other biological processes. Overall, the Uncoated Rat IL-1α ELISA Kit is a reliable and effective tool for the quantitative measurement of rat IL-1α in various sample types.