Uncoated Rat sCD86(Soluble Cluster of Differentiation 86) ELISA Kit

The Uncoated Rat sCD86 ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of soluble Cluster of Differentiation 86 (sCD86) in rat serum, plasma, and cell culture supernatants. The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of sCD86. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample, standards, and detection reagent to the wells, followed by incubation and washing steps. The amount of sCD86 is then quantified by measuring the absorbance at 450 nm using a microplate reader.

The Uncoated Rat sCD86 ELISA Kit has a detection range of 31.25-2000 pg/mL, with a sensitivity of 18.75 pg/mL. The kit has been validated for accuracy, precision, linearity, and specificity, and has a coefficient of variation (CV) of less than 10%. The kit is easy to use, with a simple protocol that can be completed in less than 3 hours. The kit includes all necessary reagents and materials, including a microplate, standards, detection reagent, wash buffer, and substrate solution. The kit is suitable for research applications in immunology, inflammation, and autoimmunity, and can be used to study the role of sCD86 in immune regulation and disease pathogenesis.