Uncoated Rat SELE(E-selectin) ELISA Kit

The Uncoated Rat SELE(E-selectin) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of rat E-selectin in serum, plasma, and cell culture supernatants. This kit is uncoated, which means that the user can coat the plate with their own capture antibody, allowing for greater flexibility in experimental design. The kit includes all necessary reagents and materials for the assay, including pre-coated plates, detection antibodies, standards, and buffers.

The assay is based on the sandwich ELISA principle, where the capture antibody is immobilized on the plate and binds to the target protein. The detection antibody, conjugated to an enzyme, is then added and binds to a different epitope on the target protein. The enzyme catalyzes a colorimetric reaction, which is measured using a microplate reader. The concentration of the target protein is determined by comparing the absorbance of the sample to a standard curve generated using known concentrations of the target protein. The kit has a detection range of 31.25-2000 pg/mL and a sensitivity of 18.75 pg/mL. This kit is ideal for researchers studying inflammation, vascular biology, and immune responses in rats.