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Elabscience
SKU(재고 관리 코드):E-UNEL-R0052
Uncoated Rat NRP2(Neuropilin 2) ELISA Kit
Uncoated Rat NRP2(Neuropilin 2) ELISA Kit
The Uncoated Rat NRP2 (Neuropilin 2) ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of Neuropilin 2 levels in rat samples. This ELISA kit is specifically developed for research purposes and provides accurate results with high sensitivity. It is an uncoated kit, meaning that the antibodies required for the assay are not pre-coated on the microplate, allowing for flexibility in experimental design and customization.
This ELISA kit utilizes a sandwich enzyme immunoassay principle, where the target protein (Neuropilin 2) is captured by a specific antibody coated on the microplate wells. After the addition of the sample and subsequent incubation, a biotinylated detection antibody is added, forming a sandwich complex with the captured protein. Streptavidin-HRP conjugate is then added, binding to the biotinylated antibody. Finally, a substrate solution is added, resulting in a color change that is directly proportional to the amount of Neuropilin 2 present in the sample. The absorbance is measured at a specific wavelength using a microplate reader, allowing for precise quantification.
This Uncoated Rat NRP2 ELISA Kit offers several advantages, including its high sensitivity, wide dynamic range, and ease of use. With a detection range of 0.156-10 ng/mL, it enables accurate measurement of both low and high levels of Neuropilin 2 in rat samples. The kit provides all the necessary components, including pre-diluted standards, detection antibodies, streptavidin-HRP conjugate, and substrate solution, ensuring convenience and reliability. Additionally, the uncoated nature of the kit allows for customization, enabling researchers to coat their own specific antibodies if desired. Overall, this ELISA kit is an indispensable tool for studying the role of Neuropilin 2 in various research areas, including neuroscience, cancer biology, and developmental biology.
This ELISA kit utilizes a sandwich enzyme immunoassay principle, where the target protein (Neuropilin 2) is captured by a specific antibody coated on the microplate wells. After the addition of the sample and subsequent incubation, a biotinylated detection antibody is added, forming a sandwich complex with the captured protein. Streptavidin-HRP conjugate is then added, binding to the biotinylated antibody. Finally, a substrate solution is added, resulting in a color change that is directly proportional to the amount of Neuropilin 2 present in the sample. The absorbance is measured at a specific wavelength using a microplate reader, allowing for precise quantification.
This Uncoated Rat NRP2 ELISA Kit offers several advantages, including its high sensitivity, wide dynamic range, and ease of use. With a detection range of 0.156-10 ng/mL, it enables accurate measurement of both low and high levels of Neuropilin 2 in rat samples. The kit provides all the necessary components, including pre-diluted standards, detection antibodies, streptavidin-HRP conjugate, and substrate solution, ensuring convenience and reliability. Additionally, the uncoated nature of the kit allows for customization, enabling researchers to coat their own specific antibodies if desired. Overall, this ELISA kit is an indispensable tool for studying the role of Neuropilin 2 in various research areas, including neuroscience, cancer biology, and developmental biology.
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