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SKU(재고 관리 코드):LMA542Ra
Magnetic Luminex Assay Kit for Endostatin (ES) ,etc.
Magnetic Luminex Assay Kit for Endostatin (ES) ,etc.
Product No.
LMA542Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.1-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.033 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Endostatin (ES) ,etc..
No significant cross-reactivity or interference between Endostatin (ES) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Endostatin (ES) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Endostatin (ES) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 78-90 | 82 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endostatin (ES) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endostatin (ES) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endostatin (ES) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-97% | 89-103% | 94-102% | 90-101% |
| EDTA plasma(n=5) | 81-98% | 78-96% | 80-92% | 92-101% |
| heparin plasma(n=5) | 78-88% | 89-97% | 97-105% | 95-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ES) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Cancer Immunology | Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice SpringerLink: u38777121t6660l5 |
| Journal of Fluoresence | Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy SpringerLink: 4v306130302257w0 |
| The Journal of Gene Medicine | Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma Wiley: source |
| Biomedicine & Pharmacotherapy | Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma ScienceDirect: S075333221100151X |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source |
| The Journal of Immunology | Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer PubMed: PMC3619527 |
| Prostaglandins & Other Lipid Mediators | Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema Pubmed:24911647 |
| American Journal of Physiology - Heart and Circulatory Physiology | Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? Source |
| shock | Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome PubMed: 26125086 |
| Clin Rev Allergy Immunol | Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066 |
| Angiologia e Cirurgia Vascular | Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052 |
| Clinical Rheumatology | Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752 |
| Blood biomarker for early blood brain barrier disruption in ischemic stroke patent:US20170199205A1 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA542Ra01 | Recombinant Endostatin (ES) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CEA542Ra | ELISA Kit for Endostatin (ES) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA542Ra | Magnetic Luminex Assay Kit for Endostatin (ES) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA542Ra11 | ELISA Kit DIY Materials for Endostatin (ES) | Main materials for "Do It (ELISA Kit) Yourself". |
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