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SKU(재고 관리 코드):LMA911Ge
Magnetic Luminex Assay Kit for Aldosterone (ALD) ,etc.
Magnetic Luminex Assay Kit for Aldosterone (ALD) ,etc.
Product No.
LMA911Ge
Organism Species
Pan-species (General).
Sample Type
serum, plasma and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
1.95-2000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.65 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Aldosterone (ALD) ,etc..
No significant cross-reactivity or interference between Aldosterone (ALD) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Aldosterone (ALD) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Aldosterone (ALD) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 94-103 | 98 |
| heparin plasma(n=5) | 78-93 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldosterone (ALD) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldosterone (ALD) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aldosterone (ALD) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-96% | 86-101% | 90-102% | 91-99% |
| EDTA plasma(n=5) | 84-101% | 95-103% | 89-102% | 88-105% |
| heparin plasma(n=5) | 81-89% | 81-105% | 87-96% | 94-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ALD) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| PLoS One | High Salt Intake Down-Regulates Colonic Mineralocorticoid Receptors, Epithelial Sodium Channels and 11β-Hydroxysteroid Dehydrogenase Type 2 PlosOne: Source |
| Physiological Reports | Evidence for glucocorticoid&-mediated hypertension after uninephrectomy Pubmed: 24303173 |
| International Journal of Endocrinology | Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach Hindawi: 384583 |
| Int J Endocrinol. | Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach. Pubmed:24672546 |
| Archives of Physiology and Biochemistry | Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364 |
| Pflügers Archiv - European Journal of Physiology | H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction. pubmed:27562425 |
| Journal of Cardiovascular Disease Research | Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertension publication:311777221 |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460 |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy pubmed:29240788 |
| Endocrinology | Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy. pubmed:29059290 |
| Journal of Thoracic and Cardiovascular Surgery | Acute adrenal cortex injury during cardiopulmonary bypass in a canine model Pubmed:29753511 |
| American Journal of Physiology-renal Physiology | ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load Pubmed: 31188029 |
| Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model Pubmed: 31540304 |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626 |
| European Journal of Pharmacolog | Preventive preclinical efficacy of intravenously administered sphingosine-1-phosphate (S1P) in strengthening hypoxia adaptive responses to acute and sub-chronic … |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990 |
| Pflugers Arch | Decreased KLHL3 expression is involved in the activation of WNK-OSR1/SPAK-NCC cascade in type 1 diabetic mice 33432425 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| MEA911Ge | Mini Samples ELISA Kit for Aldosterone (ALD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA911Ge | ELISA Kit for Aldosterone (ALD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA911Ge | Magnetic Luminex Assay Kit for Aldosterone (ALD) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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