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SKU(재고 관리 코드):LMB342Mi
Magnetic Luminex Assay Kit for Actin Alpha 2, Smooth Muscle (ACTa2) ,etc.
Magnetic Luminex Assay Kit for Actin Alpha 2, Smooth Muscle (ACTa2) ,etc.
Product No.
LMB342Mi
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat).
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.49-500ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.163 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity for detection of Actin Alpha 2, Smooth Muscle (ACTa2) ,etc..
100% cross-reactivity of Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. was observed among Human, Mouse, Rat.
Recovery
Matrices listed below were spiked with certain level of recombinant Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-104 | 87 |
| EDTA plasma(n=5) | 81-105 | 88 |
| heparin plasma(n=5) | 79-89 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Actin Alpha 2, Smooth Muscle (ACTa2) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-93% | 86-94% | 96-104% | 90-104% |
| EDTA plasma(n=5) | 85-105% | 97-105% | 80-101% | 89-103% |
| heparin plasma(n=5) | 86-101% | 88-95% | 86-94% | 92-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ACTa2) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496 |
| NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats Pubmed: 32936352 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049 |
| Clinical and Translational Medicine | NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network 34459125 |
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