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SKU(재고 관리 코드):LMA825Mu
Magnetic Luminex Assay Kit for Ischemia Modified Albumin (IMA) ,etc.
Magnetic Luminex Assay Kit for Ischemia Modified Albumin (IMA) ,etc.
Product No.
LMA825Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
29.3-30000ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 9.767 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Ischemia Modified Albumin (IMA) ,etc..
No significant cross-reactivity or interference between Ischemia Modified Albumin (IMA) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ischemia Modified Albumin (IMA) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Ischemia Modified Albumin (IMA) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-94 | 87 |
| EDTA plasma(n=5) | 83-102 | 93 |
| heparin plasma(n=5) | 83-98 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ischemia Modified Albumin (IMA) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ischemia Modified Albumin (IMA) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ischemia Modified Albumin (IMA) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 97-105% | 99-105% | 81-101% |
| EDTA plasma(n=5) | 78-102% | 88-99% | 93-101% | 83-93% |
| heparin plasma(n=5) | 98-105% | 91-101% | 79-94% | 93-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:IMA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Aging Clin Exp Res | The role of protein oxidation and DNA damage in elderly hypertension PubMed: 26487663 |
| The Anatolian Journal of Cardiology | The impact of protein oxidation on sustained and white coat hypertension pubmed:27684518 |
| Indian Journal of Thoracic and Cardiovascular Surgery | Investigation of serum ischemia-modified albumin levels in coronary artery disease patients |
| Journal of Cardiovascular Medicine | Effects of Different Heart Positions During Off-pump Coronary Artery Bypass Surgery on Serum Ischemia Modified Albumin Level and Cardiac Output |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEA825Mu | ELISA Kit for Ischemia Modified Albumin (IMA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA825Mu | ELISA Kit for Ischemia Modified Albumin (IMA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA825Mu | Magnetic Luminex Assay Kit for Ischemia Modified Albumin (IMA) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA825Mu11 | ELISA Kit DIY Materials for Ischemia Modified Albumin (IMA) | Main materials for "Do It (ELISA Kit) Yourself". |
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