Cloud-clone
SKU(재고 관리 코드):LMB390Hu
Magnetic Luminex Assay Kit for Adenosine Deaminase (ADA) ,etc.
Magnetic Luminex Assay Kit for Adenosine Deaminase (ADA) ,etc.
Product No.
LMB390Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Adenosine Deaminase (ADA) ,etc..
No significant cross-reactivity or interference between Adenosine Deaminase (ADA) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Adenosine Deaminase (ADA) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Adenosine Deaminase (ADA) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-103 | 84 |
| EDTA plasma(n=5) | 85-99 | 92 |
| heparin plasma(n=5) | 79-93 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Deaminase (ADA) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Deaminase (ADA) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adenosine Deaminase (ADA) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 93-101% | 82-101% | 85-95% |
| EDTA plasma(n=5) | 87-102% | 95-102% | 86-96% | 80-95% |
| heparin plasma(n=5) | 95-102% | 98-105% | 80-94% | 88-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ADA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Scope Med | Serum adenosine deaminase activity in Iraqi patients with breast cancer on tamoxifen therapy ScopeMed: source |
| Journal of Experimental and Integrative Medicine | Purine metabolism and oxidative stress in children with autistic spectrum disorders. Scopemed: Source |
| International Journal of Chemical and Natural Science | Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone portal:uploads |
| International Journal of Pharma Sciences | Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues portal:uploads |
| bioRxiv | Elevated serum adenosine deaminase levels in neuroleptic-naive patients with recent-onset schizophrenia S1876201816303677 |
| Asian Journal of Psychiatry | Elevated serum adenosine deaminase levels in neuroleptic-na¨ıve patients with recent-onset schizophrenia pubmed:29061410 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB390Hu01 | Recombinant Adenosine Deaminase (ADA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB390Hu01 | Polyclonal Antibody to Adenosine Deaminase (ADA) | WB; IHC; ICC; IP. |
| MAB390Hu22 | Monoclonal Antibody to Adenosine Deaminase (ADA) | WB; IHC; ICC; IP. |
| SEB390Hu | ELISA Kit for Adenosine Deaminase (ADA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB390Hu | Magnetic Luminex Assay Kit for Adenosine Deaminase (ADA) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
