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SKU(재고 관리 코드):LMA085Ra
Magnetic Luminex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc.
Magnetic Luminex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc.
Product No.
LMA085Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Leukemia Inhibitory Factor (LIF) ,etc..
No significant cross-reactivity or interference between Leukemia Inhibitory Factor (LIF) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Leukemia Inhibitory Factor (LIF) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Leukemia Inhibitory Factor (LIF) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-101 | 86 |
| EDTA plasma(n=5) | 80-92 | 81 |
| heparin plasma(n=5) | 97-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukemia Inhibitory Factor (LIF) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukemia Inhibitory Factor (LIF) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leukemia Inhibitory Factor (LIF) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 85-94% | 93-105% | 84-98% |
| EDTA plasma(n=5) | 85-101% | 94-104% | 80-94% | 83-96% |
| heparin plasma(n=5) | 95-102% | 86-101% | 88-96% | 78-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:LIF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| British Journal of Anaesthesia | Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids pubmed:28077540 |
| Biological Rhythm Research | A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159 |
| Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA085Ra01 | Recombinant Leukemia Inhibitory Factor (LIF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA085Ra01 | Active Leukemia Inhibitory Factor (LIF) | Cell culture; Activity Assays. |
| PAA085Ra01 | Polyclonal Antibody to Leukemia Inhibitory Factor (LIF) | WB; IHC; ICC; IP. |
| MAA085Ra21 | Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) | WB; IHC; ICC; IP. |
| SEA085Ra | ELISA Kit for Leukemia Inhibitory Factor (LIF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA085Ra | Magnetic Luminex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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