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SKU(재고 관리 코드):LMA896Mu
Magnetic Luminex Assay Kit for Cystatin C (Cys-C) ,etc.
Magnetic Luminex Assay Kit for Cystatin C (Cys-C) ,etc.
Product No.
LMA896Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Cystatin C (Cys-C) ,etc..
No significant cross-reactivity or interference between Cystatin C (Cys-C) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cystatin C (Cys-C) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Cystatin C (Cys-C) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-105 | 102 |
| EDTA plasma(n=5) | 83-96 | 86 |
| heparin plasma(n=5) | 82-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin C (Cys-C) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin C (Cys-C) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cystatin C (Cys-C) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 98-105% | 80-93% | 78-92% |
| EDTA plasma(n=5) | 96-105% | 87-101% | 90-98% | 89-98% |
| heparin plasma(n=5) | 96-104% | 82-101% | 78-99% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:Cys-C) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| BioMed Research International | The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105 |
| Critical Care | Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding protein Pubmed:25261195 |
| РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ 616-005.8 | |
| Journal of Agricultural and Food Chemistry | Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice pubmed:29254330 |
| THERAPEUTIC ADVANCES IN RESPIRATORY DISEASE | Association of cathepsin B and cystatin C with an age-related pulmonary subclinical state in a healthy Chinese population Pubmed: 32401159 |
| RSC Adv | SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury Pubmed:35733690 |
| Food Funct | A Compared Study of Gentle Ketogenic Diet Containing Medium-Chain Triglycerides or Long-Chain Triglycerides on Chronic Sleep Deprivation-Induced Cognitive … Pubmed:35141738 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA896Mu01 | Recombinant Cystatin C (Cys-C) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA896Mu02 | Recombinant Cystatin C (Cys-C) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA896Mu02 | Polyclonal Antibody to Cystatin C (Cys-C) | WB; IHC; ICC; IP. |
| PAA896Mu01 | Polyclonal Antibody to Cystatin C (Cys-C) | WB; IHC; ICC; IP. |
| SEA896Mu | ELISA Kit for Cystatin C (Cys-C) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA896Mu | Magnetic Luminex Assay Kit for Cystatin C (Cys-C) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA896Mu01 | ELISA Kit DIY Materials for Cystatin C (Cys-C) | Main materials for "Do It (ELISA Kit) Yourself". |
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