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SKU(재고 관리 코드):LMC038Ge
Magnetic Luminex Assay Kit for Diacylglycerol (DAG) ,etc.
Magnetic Luminex Assay Kit for Diacylglycerol (DAG) ,etc.
Product No.
LMC038Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
97.66-100000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 32.553 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Diacylglycerol (DAG) ,etc..
No significant cross-reactivity or interference between Diacylglycerol (DAG) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Diacylglycerol (DAG) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Diacylglycerol (DAG) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-97 | 92 |
| EDTA plasma(n=5) | 87-94 | 90 |
| heparin plasma(n=5) | 84-102 | 98 |
| sodium citrate plasma(n=5) | 84-98 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol (DAG) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol (DAG) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Diacylglycerol (DAG) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 89-105% | 83-92% | 88-98% |
| EDTA plasma(n=5) | 78-102% | 97-105% | 79-101% | 78-97% |
| heparin plasma(n=5) | 78-102% | 91-98% | 97-104% | 91-101% |
| sodium citrate plasma(n=5) | 92-101% | 88-97% | 85-93% | 88-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:DAG) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Acta Pharmacologica Sinica | Wogonin ameliorates lipotoxicity-induced apoptosis of cultured vascular smooth muscle cells via interfering with DAG-PKC pathway Nature: 2011120a |
| Psychoneuroendocrinology | Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539 |
| The Journal of Biological Chemistry | Aryl Hydrocarbon Receptor Plays Protective Roles against High Fat Diet (HFD)-induced Hepatic Steatosis and the Subsequent Lipotoxicity via Direct Transcriptional ReCavia (Guinea pig )lation of Socs3 Gene Expression Pubmed:26865635 |
| Journal of the American Heart Association | Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R). pubmed:27413044 |
| International Journal of Molecular Medicine | PEDF protects cardiomyocytes by promoting FUNDC1‑mediated mitophagy via PEDF-R under hypoxic condition Pubmed:29512692 |
| British Journal of Pharmacology | Bouchardatine analogue alleviates NAFLD/NASH in high fat fed mice via blunting ATP synthase activity Pubmed: 31113010 |
| Gut Akkermansia muciniphila ameliorates non-alcoholic fatty liver disease by L-aspartate via interaction with liver | |
| Histochem Cell Biol | The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats 34312706 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPC038Ge11 | BSA Conjugated Diacylglycerol (DAG) | Immunogen; SDS-PAGE; WB. |
| CPC038Ge21 | OVA Conjugated Diacylglycerol (DAG) | Immunogen; SDS-PAGE; WB. |
| PAC038Ge01 | Polyclonal Antibody to Diacylglycerol (DAG) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAC038Ge71 | Biotin-Linked Polyclonal Antibody to Diacylglycerol (DAG) | WB; IHC; ICC. |
| CEC038Ge | ELISA Kit for Diacylglycerol (DAG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC038Ge | Magnetic Luminex Assay Kit for Diacylglycerol (DAG) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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