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SKU(재고 관리 코드):LMB863Ge
Magnetic Luminex Assay Kit for Nitrotyrosine (NT) ,etc.
Magnetic Luminex Assay Kit for Nitrotyrosine (NT) ,etc.
Product No.
LMB863Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.29-300ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.097 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Nitrotyrosine (NT) ,etc..
No significant cross-reactivity or interference between Nitrotyrosine (NT) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Nitrotyrosine (NT) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Nitrotyrosine (NT) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-104 | 98 |
| EDTA plasma(n=5) | 87-94 | 91 |
| heparin plasma(n=5) | 87-101 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitrotyrosine (NT) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitrotyrosine (NT) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nitrotyrosine (NT) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-95% | 96-105% | 96-105% | 91-99% |
| EDTA plasma(n=5) | 82-101% | 93-102% | 79-94% | 87-96% |
| heparin plasma(n=5) | 99-105% | 85-101% | 94-102% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NT) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Molecular Medicine Reports | 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat model Pubmed: 23970331 |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835 |
| J Histochem Cytochem | Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats PubMed: 26385569 |
| canadian journal of physiology and pharmacology | Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor 10.1139:cjpp-2017-0042 |
| Orphanet journal of rare diseases | The cardiovascular phenotype of adult patients with phenylketonuria Pubmed: 31492166 |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPB863Ge11 | BSA Conjugated Nitrotyrosine (NT) | Immunogen; SDS-PAGE; WB. |
| CPB863Ge21 | OVA Conjugated Nitrotyrosine (NT) | Immunogen; SDS-PAGE; WB. |
| PAB863Ge01 | Polyclonal Antibody to Nitrotyrosine (NT) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEB863Ge | ELISA Kit for Nitrotyrosine (NT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB863Ge | Magnetic Luminex Assay Kit for Nitrotyrosine (NT) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB863Ge11 | ELISA Kit DIY Materials for Nitrotyrosine (NT) | Main materials for "Do It (ELISA Kit) Yourself". |
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