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SKU(재고 관리 코드):LMC842Hu
Magnetic Luminex Assay Kit for Secreted Frizzled Related Protein 5 (SFRP5) ,etc.
Magnetic Luminex Assay Kit for Secreted Frizzled Related Protein 5 (SFRP5) ,etc.
Product No.
LMC842Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.1-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.033 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5) ,etc..
No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Secreted Frizzled Related Protein 5 (SFRP5) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Secreted Frizzled Related Protein 5 (SFRP5) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-104 | 94 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 97-105 | 101 |
| sodium citrate plasma(n=5) | 79-101 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secreted Frizzled Related Protein 5 (SFRP5) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-104% | 94-101% | 97-104% | 81-93% |
| EDTA plasma(n=5) | 84-96% | 87-104% | 93-101% | 93-101% |
| heparin plasma(n=5) | 93-103% | 82-90% | 98-105% | 84-102% |
| sodium citrate plasma(n=5) | 88-102% | 92-99% | 99-105% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SFRP5) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Diabetes Research and Clinical Practice | Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance PubMed: 23290274 |
| European Journal of Clinical Investigation | Sfrp5 correlates with insulin resistance and oxidative stress PubMed: 23398169 |
| Pediatric Research | Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children Pubmed: 24603290 |
| Clin Endocrinol (Oxf). | SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children Pubmed: 24330025 |
| The Journal of Clinical Endocrinology & Metabolism | Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans Pubmed: 23185036 |
| Atherosclerosis | Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease Pubmed: 24530778 |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation Pubmed:Pmc4072672 |
| J Clin Endocrinol Metab. | Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation Pubmed:24840810 |
| Oncology Letters | Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma Pubmed:25120720 |
| Diabetes Metab Res Rev. | Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls Pubmed:25139699 |
| Clinical & Experimental Immunology | The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis Pubmed:25382802 |
| Diabetes Metab Res Rev | Circulating SFRP5 levels are elevated in drug-na PubMed: 25139699 |
| J Matern Fetal Neonatal Med | Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels PubMed: 26100762 |
| Eur J Clin Invest | Sfrp5 associates with beta-cell function in humans Pubmed:27019073 |
| Osteoporosis International | Bone marrow sFRP5 level is negatively associated with bone formation markers pubmed:27986984 |
| Diabetes/Metabolism Research and Reviews | Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults pubmed:28303637 |
| Cardiovascular Diabetology | Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study pubmed:28851362 |
| Arteriosclerosis, Thrombosis, and Vascular Biology | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … Pubmed:29674475 |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376 |
| J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats article-1-352-en.pdf |
| Molecular Biology Reports | The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome Pubmed: 30194556 |
| Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling | |
| British Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290 |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans Pubmed: 30137542 |
| Frontiers in Endocrinology | Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients Pubmed: 32300333 |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease Pubmed: 33188773 |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … Pubmed: 32491086 |
| Kidney Blood Press Res | Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway 34469882 |
| Cytokine | Association of serum uromodulin with adipokines in dependence of type 2 diabetes 34920231 |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470 |
| JMIR Res Protoc | Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition … Pubmed:35475790 |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop … |
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