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SKU(재고 관리 코드):LMB019Ra
Magnetic Luminex Assay Kit for Angiopoietin Like Protein 4 (ANGPTL4) ,etc.
Magnetic Luminex Assay Kit for Angiopoietin Like Protein 4 (ANGPTL4) ,etc.
Product No.
LMB019Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.1-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.033 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 4 (ANGPTL4) ,etc..
No significant cross-reactivity or interference between Angiopoietin Like Protein 4 (ANGPTL4) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Angiopoietin Like Protein 4 (ANGPTL4) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Angiopoietin Like Protein 4 (ANGPTL4) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-96 | 93 |
| EDTA plasma(n=5) | 78-96 | 86 |
| heparin plasma(n=5) | 91-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin Like Protein 4 (ANGPTL4) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-103% | 78-104% | 82-94% | 84-101% |
| EDTA plasma(n=5) | 85-96% | 88-97% | 91-105% | 85-101% |
| heparin plasma(n=5) | 79-95% | 87-94% | 85-94% | 89-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ANGPTL4) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| The Journal of Biological Chemistry | Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes PubMed: PMC3318686 |
| J Clin Endocrinol Metab. | Decreased Concentrations of the Lipoprotein Lipase Inhibitor Angiopoietin-Like Protein 4 and Increased Serum Triacylglycerol Are Associated With Increased Neonatal Fat Mass in Pregnant Women With Gestational Diabetes Mellitus Pubmed: 23744407 |
| PloS one | Lack of Relationship between Cord Serum Angiopoietin-Like Protein 4 (ANGPTL4) and Lipolytic Activity in Human Neonates Born by Spontaneous Delivery Pubmed: 24324678 |
| Journal of Hematology & Oncology | Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy PubMed: 26054961 |
| Gynecol Endocrinol | Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome PubMed: 26291814 |
| Molecular Nutrition & Food research | Interaction of Gut Microbiota and High‐sodium, Low‐potassium Diet in Altering Plasma Triglyceride Profiles Revealed by Lipidomics Analysis Pubmed: 31675161 |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis |
| Food Chem Toxicol | Di (2-ethylhexyl) phthalate exposure exacerbates metabolic disorders in diet-induced obese mice 34303773 |
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