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SKU(재고 관리 코드):LMA105Hu
Magnetic Luminex Assay Kit for Nerve Growth Factor (NGF) ,etc.
Magnetic Luminex Assay Kit for Nerve Growth Factor (NGF) ,etc.
Product No.
LMA105Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF) ,etc..
No significant cross-reactivity or interference between Nerve Growth Factor (NGF) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Nerve Growth Factor (NGF) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Nerve Growth Factor (NGF) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-105 | 98 |
| EDTA plasma(n=5) | 84-92 | 88 |
| heparin plasma(n=5) | 91-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor (NGF) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor (NGF) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nerve Growth Factor (NGF) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-103% | 80-94% | 98-105% | 92-101% |
| EDTA plasma(n=5) | 81-92% | 90-104% | 80-93% | 78-102% |
| heparin plasma(n=5) | 78-97% | 79-96% | 91-105% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NGF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| The Tohoku Journal of Experimental Medicine | Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats Pubmed:25212248 |
| International Journal of Pharmaceutics | Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs Pubmed:25445537 |
| Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome Pubmed:25510766 |
| Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 Pubmed:25614971 |
| Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 PubMed: 25614971 |
| Neurosciences (Riyadh) | Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. PubMed: 25864063 |
| Neurotoxicity Research | Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures Pubmed:27189478 |
| Psychoneuroendocrinology | Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism Pubmed:26771945 |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639 |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease |
| biblioteca.uva | Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo 10324/27440 |
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| Pharmacological Research | N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442 |
| Experimental Neurology | Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511 |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats Pubmed:30028991 |
| Experimental Dermatology | 3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress Pubmed: 30698879 |
| BioMed Research International | Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807 |
| Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Pubmed: 32010279 |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy Pubmed: 32165929 |
| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … Pubmed: 32918393 |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084 |
| COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease Pubmed: 32777662 |
| 博士论文 | Neurobiological determinants of depressive-like symptoms in rodents |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy |
| Journal of the Taiwan Institute of Chemical Engineers | Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
| DRUG DEVELOPMENT RESEARCH | Neuroprotective effect of Na+/H+?exchangers isoform©\1 inactivation against 6©\hydroxydopamine©\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models 33538000 |
| BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896 |
| Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … 34239303 |
| Pharmaceuticals (Basel) | Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers 34959705 |
| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway 34296452 |
| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration Pubmed:35550777 |
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