Cloud-clone
SKU(재고 관리 코드):LMA655Ra
Magnetic Luminex Assay Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc.
Magnetic Luminex Assay Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc.
Product No.
LMA655Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc..
No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-94 | 87 |
| EDTA plasma(n=5) | 83-97 | 87 |
| heparin plasma(n=5) | 85-99 | 94 |
| sodium citrate plasma(n=5) | 97-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-97% | 95-102% | 80-89% | 87-101% |
| EDTA plasma(n=5) | 89-96% | 83-105% | 89-99% | 87-98% |
| heparin plasma(n=5) | 80-102% | 91-98% | 91-99% | 89-97% |
| sodium citrate plasma(n=5) | 85-103% | 87-96% | 90-104% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CAMK2g) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| PLOS ONE | Role of Calmodulin-Calmodulin Kinase II, cAMP/Protein Kinase A and ERK 1/2 on Aeromonas hydrophila-Induced Apoptosis of Head Kidney Macrophages Pubmed:24763432 |
| PLoS One | Calcium and Superoxide-Mediated Pathways Converge to Induce Nitric Oxide-Dependent Apoptosis in Mycobacterium fortuitum-Infected Fish Macrophages Pubmed:26752289 |
| Toxicology Research | Fluoride-induced headkidney macrophage cell apoptosis involves activation of the CaMKIIg-ERK 1/2-caspase-8 axis: the role of superoxide in initiating the apoptotic cascade C6TX00206D#!divAbstract |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA655Ra01 | Recombinant Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA655Ra01 | Polyclonal Antibody to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) | WB,IHC |
| MAA655Ra21 | Monoclonal Antibody to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) | WB; IHC; ICC; IP. |
| SEA655Ra | ELISA Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA655Ra | Magnetic Luminex Assay Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
