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LMG409Ge

Magnetic Luminex Assay Kit for Nicotinamide Adenine Dinucleotide (NAD) ,etc.

Magnetic Luminex Assay Kit for Nicotinamide Adenine Dinucleotide (NAD) ,etc.

Detection range
Size
Sensitivity

Product No.

LMG409Ge

Organism Species

Pan-species (General).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Competitive Inhibition

Assay Length

1.5h

Detection Range

9.77-10000ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 3.257 ng/mL.

UOM

1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide (NAD) ,etc..
No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide (NAD) ,etc. and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Nicotinamide Adenine Dinucleotide (NAD) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Nicotinamide Adenine Dinucleotide (NAD) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-101 86
EDTA plasma(n=5) 78-91 84
heparin plasma(n=5) 91-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide (NAD) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide (NAD) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nicotinamide Adenine Dinucleotide (NAD) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 78-104% 92-103% 82-101%
EDTA plasma(n=5) 80-101% 82-99% 80-92% 80-96%
heparin plasma(n=5) 78-95% 84-95% 85-93% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NAD) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

     

    Magazine Citations
    neurotoxicology Targeting sirtuin activity with nicotinamide riboside reduces neuroinflammation in a GWI mouse model Pubmed: 32343995
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