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SKU(재고 관리 코드):LMB203Ra
Magnetic Luminex Assay Kit for Neurotensin (NT) ,etc.
Magnetic Luminex Assay Kit for Neurotensin (NT) ,etc.
Product No.
LMB203Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
2.93-3000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.977 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurotensin (NT) ,etc..
No significant cross-reactivity or interference between Neurotensin (NT) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Neurotensin (NT) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotensin (NT) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-101 | 97 |
| EDTA plasma(n=5) | 81-89 | 85 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotensin (NT) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotensin (NT) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotensin (NT) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 86-94% | 78-101% | 89-101% |
| EDTA plasma(n=5) | 91-98% | 80-90% | 90-98% | 85-98% |
| heparin plasma(n=5) | 91-99% | 92-101% | 84-91% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NT) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation pubmed:29082242 |
| Molecular Neurobiology | Inhibition of Peroxynitrite-induced mitophagy activation attenuates cerebral ischemia-reperfusion injury Pubmed:29307080 |
| Disease Markers | Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS |
| Digestive and Liver Disease | Functional analysis of gut microbiota and immunoinflammation in children with autism spectrum disorders Pubmed: 31320306 |
| Biomolecules | Association between Circular RNA CDR1as and Post-Infarction Cardiac Function in Pig Ischemic Heart Failure: Influence of the Anti-Fibrotic Natural Compounds … Pubmed: 32823854 |
| Biomed Research International | Qiliqiangxin Improves Cardiac Function through Regulating Energy Metabolism via HIF-1α-Dependent and Independent Mechanisms in Heart Failure Rats … Pubmed: 32626732 |
| Nutrition, Metabolism and Cardiovascular Diseases | Identification of U-shaped Curve Relation in Pro-neurotensin and Risk of Coronary Artery Disease (CAD) in Premature CAD Patients |
| Effect of dobutamine on intrinsic myocardial function and myocardial apoptosis in septic rats with myocardial dysfunction | |
| ACTA PHARMACOLOGICA SINICA | Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy 33785860 |
| J Cell Mol Med | Elabela gene therapy promotes angiogenesis after myocardial infarction 34291565 |
| Medicina (Kaunas) | Renal Impairment Detectors: IGFBP-7 and NGAL as Tubular Injury Markers in Multiple Myeloma Patients 34946293 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB203Ra01 | Recombinant Neurotensin (NT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB203Ra21 | OVA Conjugated Neurotensin (NT) | Immunogen; SDS-PAGE; WB. |
| CPB203Ra22 | OVA Conjugated Neurotensin (NT) | Immunogen; SDS-PAGE; WB. |
| PAB203Ra01 | Polyclonal Antibody to Neurotensin (NT) | WB; IHC; ICC; IP. |
| MAB203Ra21 | Monoclonal Antibody to Neurotensin (NT) | WB; IHC; ICC; IP. |
| CEB203Ra | ELISA Kit for Neurotensin (NT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB203Ra | Magnetic Luminex Assay Kit for Neurotensin (NT) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB203Ra11 | ELISA Kit DIY Materials for Neurotensin (NT) | Main materials for "Do It (ELISA Kit) Yourself". |
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