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SKU(재고 관리 코드):LMJ557Ra
Magnetic Luminex Assay Kit for Beclin 1 (BECN1) ,etc.
Magnetic Luminex Assay Kit for Beclin 1 (BECN1) ,etc.
Product No.
LMJ557Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Beclin 1 (BECN1) ,etc..
No significant cross-reactivity or interference between Beclin 1 (BECN1) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Beclin 1 (BECN1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Beclin 1 (BECN1) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-103 | 101 |
| EDTA plasma(n=5) | 80-94 | 88 |
| heparin plasma(n=5) | 90-101 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beclin 1 (BECN1) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beclin 1 (BECN1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beclin 1 (BECN1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 94-105% | 84-99% | 78-94% |
| EDTA plasma(n=5) | 94-101% | 96-103% | 95-103% | 81-99% |
| heparin plasma(n=5) | 88-102% | 89-98% | 85-94% | 80-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:BECN1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Rejuvenation Res. | Can Enhanced Autophagy be Associated with Human Longevity? Serum Levels of the Autophagy Biomarker Beclin-1 are Increased in Healthy Centenarians Pubmed:25307612 |
| J Cosmet Dermatol | A peel-off facial mask comprising myoinositol and trehalose-loaded liposomes improves adult female acne by reducing local hyperandrogenism and activating autophagy pubmed:28342238 |
| Tumor Biology | The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical models pubmed:28639900 |
| 19 | Beclin1 circulating levels and accelerated ageing markers in COPD DOI: 10.1183/1393003.congress-2017.PA1018 |
| Cell Death & Disease | Beclin1 circulating levels and accelerated aging markers in COPD Pubmed:29402890 |
| Clinica Chimica Acta | A novel combined score of biomarkers in sputum may be an indicator for lung cancer: A pilot study Doi: 10.1016/j.cca.2018.09.027 |
| International Journal of Clinical and Experimental Pathology | Serum concentration of autophagy-related protein 7 is correlated with non-alcoholic fatty liver disease in Chinese adults |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study 33936265 |
| International Journal for Research in Applied Sciences and Biotechnology | Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N (4)-Methylthiosemicarbazone |
| BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY?¡ | |
| European Journal of Natural History | IDENTIFICATION OF FACTORS CONNECTED WITH AUTOPHAGY ACTIVITY UNDER CONDITIONS OF PARTIAL NUTRITIONAL DEPRIVATION IN MEN |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPJ557Ra01 | Recombinant Beclin 1 (BECN1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ557Ra01 | Polyclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| MAJ557Ra21 | Monoclonal Antibody to Beclin 1 (BECN1) | WB; IHC; ICC; IP. |
| SEJ557Ra | ELISA Kit for Beclin 1 (BECN1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ557Ra | Magnetic Luminex Assay Kit for Beclin 1 (BECN1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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