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SKU(재고 관리 코드):LMB302Ra
Magnetic Luminex Assay Kit for Agouti Related Protein (AGRP) ,etc.
Magnetic Luminex Assay Kit for Agouti Related Protein (AGRP) ,etc.
Product No.
LMB302Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.59-600pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.197 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Agouti Related Protein (AGRP) ,etc..
No significant cross-reactivity or interference between Agouti Related Protein (AGRP) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Agouti Related Protein (AGRP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Agouti Related Protein (AGRP) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 82-102 | 98 |
| heparin plasma(n=5) | 89-103 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Agouti Related Protein (AGRP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Agouti Related Protein (AGRP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Agouti Related Protein (AGRP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-103% | 90-98% | 80-94% | 80-101% |
| EDTA plasma(n=5) | 81-103% | 86-96% | 89-98% | 80-98% |
| heparin plasma(n=5) | 85-101% | 95-102% | 88-96% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:AGRP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Проблеми еколог | МЕХАН Pdf: Mag3 |
| Світ медицини та біології. | ВИЗНАЧЕННЯ ВЗАЄМОЗВ’ЯЗКІВ ФАКТОРІВ ФОРМУВАННЯ ПІДВИЩЕНОЇ МАСИ ТІЛА У МОЛОДИХ ОСІБ SMB-2016-01-103.pdf |
| J Endocrinol Invest | Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB302Ra01 | Recombinant Agouti Related Protein (AGRP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB302Ra02 | Recombinant Agouti Related Protein (AGRP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB302Ra02 | Polyclonal Antibody to Agouti Related Protein (AGRP) | WB; IHC; ICC; IP. |
| PAB302Ra01 | Polyclonal Antibody to Agouti Related Protein (AGRP) | WB; IHC; ICC; IP. |
| LAB302Ra71 | Biotin-Linked Polyclonal Antibody to Agouti Related Protein (AGRP) | WB; IHC; ICC. |
| SEB302Ra | ELISA Kit for Agouti Related Protein (AGRP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB302Ra | Magnetic Luminex Assay Kit for Agouti Related Protein (AGRP) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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