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Magnetic Luminex Assay Kit for Hemoglobin Delta (HBd) ,etc.
Magnetic Luminex Assay Kit for Hemoglobin Delta (HBd) ,etc.
Product No.
LMD092Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, erythrocyte lysates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.2-200ug/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.067 ug/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemoglobin Delta (HBd) ,etc..
No significant cross-reactivity or interference between Hemoglobin Delta (HBd) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Hemoglobin Delta (HBd) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Hemoglobin Delta (HBd) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-97 | 91 |
| EDTA plasma(n=5) | 80-103 | 87 |
| heparin plasma(n=5) | 86-103 | 99 |
| sodium citrate plasma(n=5) | 98-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Delta (HBd) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Delta (HBd) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemoglobin Delta (HBd) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 80-103% | 98-105% | 93-102% |
| EDTA plasma(n=5) | 88-95% | 89-103% | 97-105% | 92-99% |
| heparin plasma(n=5) | 79-101% | 95-105% | 88-102% | 81-91% |
| sodium citrate plasma(n=5) | 81-102% | 80-101% | 83-90% | 94-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:HBd) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | Proteomic Analysis of Amniotic Fluid Proteins for Predicting the Outcome of Emergency Cerclage in Women with Cervical Insufficiency Pubmed: 30076666 |
| PeerJ | Antibacterial activity of human defensins against Staphylococcus aureus and Escherichia coli 33304659 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD092Hu01 | Recombinant Hemoglobin Delta (HBd) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD092Hu01 | Polyclonal Antibody to Hemoglobin Delta (HBd) | WB,IHC,ICC/IF |
| CED092Hu | ELISA Kit for Hemoglobin Delta (HBd) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD092Hu | Magnetic Luminex Assay Kit for Hemoglobin Delta (HBd) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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