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SKU(재고 관리 코드):LMB040Mu
Magnetic Luminex Assay Kit for Vimentin (VIM) ,etc.
Magnetic Luminex Assay Kit for Vimentin (VIM) ,etc.
Product No.
LMB040Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Vimentin (VIM) ,etc..
No significant cross-reactivity or interference between Vimentin (VIM) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Vimentin (VIM) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Vimentin (VIM) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-98 | 94 |
| EDTA plasma(n=5) | 94-102 | 97 |
| heparin plasma(n=5) | 85-104 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vimentin (VIM) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vimentin (VIM) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vimentin (VIM) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 93-105% | 91-101% | 95-105% |
| EDTA plasma(n=5) | 93-101% | 98-105% | 96-104% | 96-105% |
| heparin plasma(n=5) | 86-101% | 98-105% | 79-96% | 91-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:VIM) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Cancer Epidemiology, Biomarkers & Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer pubmed:22315367 |
| Scientific Reports | Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. pubmed:27898103 |
| Journal of Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin Pubmed:29477555 |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328 |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts Pubmed: 31729155 |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? Pubmed: 32088750 |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221 |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996 |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB040Mu02 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB040Mu01 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB040Mu02 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| PAB040Mu01 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| SEB040Mu | ELISA Kit for Vimentin (VIM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB040Mu | Magnetic Luminex Assay Kit for Vimentin (VIM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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