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SKU(재고 관리 코드):LMA103Hu
Magnetic Luminex Assay Kit for Matrix Metalloproteinase 8 (MMP8) ,etc.
Magnetic Luminex Assay Kit for Matrix Metalloproteinase 8 (MMP8) ,etc.
Product No.
LMA103Hu
Organism Species
Homo sapiens (Human).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
4.88-5000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.627 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 8 (MMP8) ,etc..
No significant cross-reactivity or interference between Matrix Metalloproteinase 8 (MMP8) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 8 (MMP8) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 8 (MMP8) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-102 | 98 |
| EDTA plasma(n=5) | 78-103 | 91 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 8 (MMP8) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 80-104% | 88-101% | 82-103% |
| EDTA plasma(n=5) | 79-91% | 78-91% | 93-105% | 78-97% |
| heparin plasma(n=5) | 80-99% | 79-91% | 85-99% | 83-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:MMP8) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| Journal of Periodontology | Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464 |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| An Experimental Model for Inducing Periodontal Pathology in Rat: Histopathological and Enzymatic Aspects Usamvcluj:Source | |
| Journal of Molecular and Cellular Cardiology | Treatment with anti-RANKL antibody reduces infarct size and attenuates dysfunction impacting on neutrophil-mediated injury Pubmed:27056420 |
| J Periodontal Implant Sci | Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol pmc:PMC4848383 |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| Eur J Clin Invest. | Alamandine abrogates neutrophil degranulation in atherosclerotic mice. pubmed:27930810 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010 |
| Scientific Reports | Atherosclerotic plaque vulnerability is increased in mouse model of lupus Pubmed: 33110193 |
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