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SKU(재고 관리 코드):LMC047Mu
Magnetic Luminex Assay Kit for Ki-67 Protein (Ki-67) ,etc.
Magnetic Luminex Assay Kit for Ki-67 Protein (Ki-67) ,etc.
Product No.
LMC047Mu
Organism Species
Mus musculus (Mouse).
Sample Type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.02-20ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.007 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki-67) ,etc..
No significant cross-reactivity or interference between Ki-67 Protein (Ki-67) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ki-67 Protein (Ki-67) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Ki-67 Protein (Ki-67) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-101 | 97 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 94-101 | 98 |
| sodium citrate plasma(n=5) | 94-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ki-67 Protein (Ki-67) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 98-105% | 98-105% | 79-90% |
| EDTA plasma(n=5) | 82-94% | 80-99% | 80-96% | 78-101% |
| heparin plasma(n=5) | 82-89% | 88-104% | 94-101% | 92-105% |
| sodium citrate plasma(n=5) | 96-103% | 84-91% | 78-97% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:Ki-67) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| BMC Research Notes | Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma Pubmed: 24393368 |
| BMC Research Notes | Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinoma Pubmed:24393368 |
| Chemistry & Biodiversity | Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic … Pubmed:29874411 |
| Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation | |
| Carbohydrate Polymers | A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathway Pubmed: 32299547 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC047Mu01 | Recombinant Ki-67 Protein (Ki-67) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC047Mu02 | Recombinant Ki-67 Protein (Ki-67) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC047Mu02 | Polyclonal Antibody to Ki-67 Protein (Ki-67) | WB; IHC; ICC; IP. |
| PAC047Mu01 | Polyclonal Antibody to Ki-67 Protein (Ki-67) | WB; IHC; ICC; IP. |
| SEC047Mu | ELISA Kit for Ki-67 Protein (Ki-67) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC047Mu | Magnetic Luminex Assay Kit for Ki-67 Protein (Ki-67) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSC047Mu01 | ELISA Kit DIY Materials for Ki-67 Protein (Ki-67) | Main materials for "Do It (ELISA Kit) Yourself". |
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