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SKU(재고 관리 코드):LMA393Ca
Magnetic Luminex Assay Kit for Substance P (SP) ,etc.
Magnetic Luminex Assay Kit for Substance P (SP) ,etc.
Product No.
LMA393Ca
Organism Species
Canis familiaris; Canine (Dog).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Substance P (SP) ,etc..
No significant cross-reactivity or interference between Substance P (SP) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Substance P (SP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Substance P (SP) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-101 | 96 |
| EDTA plasma(n=5) | 80-97 | 86 |
| heparin plasma(n=5) | 80-94 | 87 |
| sodium citrate plasma(n=5) | 92-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Substance P (SP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Substance P (SP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Substance P (SP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 90-98% | 78-96% | 81-90% |
| EDTA plasma(n=5) | 92-101% | 97-104% | 91-103% | 78-98% |
| heparin plasma(n=5) | 96-104% | 98-105% | 78-104% | 90-103% |
| sodium citrate plasma(n=5) | 84-92% | 78-104% | 83-104% | 86-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Journal of Veterinary Internal Medicine | A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic Pain Pubmed: 23659719 |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921 |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3 |
| CNS Neuroscience & Therapeutics | Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation Pubmed:29900692 |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement Pubmed: 30562932 |
| Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455 |
| Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model Pubmed: 33014123 |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy |
| Int Urol Nephrol | Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis 34213714 |
| J Chem Neuroanat | Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice 34626771 |
| Catalog No. | Related products for research use of Canis familiaris; Canine (Dog) Organism species | Applications (RESEARCH USE ONLY!) |
| CEA393Ca | ELISA Kit for Substance P (SP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA393Ca | CLIA Kit for Substance P (SP) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA393Ca | Magnetic Luminex Assay Kit for Substance P (SP) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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