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SKU(재고 관리 코드):LMD949Hu

Magnetic Luminex Assay Kit for RAR Related Orphan Receptor Gamma (RORg) ,etc.

Magnetic Luminex Assay Kit for RAR Related Orphan Receptor Gamma (RORg) ,etc.

Detection range
Size
Sensitivity

Product No.

LMD949Hu

Organism Species

Homo sapiens (HumanSame name, Different species.

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3.5h

Detection Range

0.01-10ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.003 ng/mL.

UOM

1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex

 

Specificity

This assay has high sensitivity and excellent specificity for detection of RAR Related Orphan Receptor Gamma (RORg) ,etc..
No significant cross-reactivity or interference between RAR Related Orphan Receptor Gamma (RORg) ,etc. and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant RAR Related Orphan Receptor Gamma (RORg) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of RAR Related Orphan Receptor Gamma (RORg) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-105 99
EDTA plasma(n=5) 86-104 89
heparin plasma(n=5) 81-94 88
sodium citrate plasma(n=5) 81-89 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAR Related Orphan Receptor Gamma (RORg) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAR Related Orphan Receptor Gamma (RORg) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RAR Related Orphan Receptor Gamma (RORg) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-103% 87-103% 94-103% 83-90%
EDTA plasma(n=5) 80-96% 96-103% 80-103% 94-103%
heparin plasma(n=5) 78-93% 82-101% 81-89% 79-102%
sodium citrate plasma(n=5) 78-98% 80-99% 78-93% 93-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:RORg) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

 

Magazine Citations
Biomed Research International Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis Pubmed: 32908870
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD949Hu01 Recombinant RAR Related Orphan Receptor Gamma (RORg) Positive Control; Immunogen; SDS-PAGE; WB.
PAD949Hu01 Polyclonal Antibody to RAR Related Orphan Receptor Gamma (RORg) WB; ICC; IHC-P; IHC-F; ELISA.
MAD949Hu21 Monoclonal Antibody to RAR Related Orphan Receptor Gamma (RORg) WB; IHC; ICC; IP.
SED949Hu ELISA Kit for RAR Related Orphan Receptor Gamma (RORg) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD949Hu Magnetic Luminex Assay Kit for RAR Related Orphan Receptor Gamma (RORg) ,etc. Magnetic Luminex Assay for Antigen Detection.
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